李菁 副教授

李菁，生命科学学院副教授，博士生导师。2011年于南京大学获理学博士学位。2011-2017年任南京大学助理研究员。2017年起担任南京大学副教授。2019年赴剑桥大学Institute of Metabolic Science访问一年。

主要方向一：糖脂稳态调节及其代际传递与调控机制

1. 新型分泌因子RNAkine在糖脂稳态调节中的生理病理作用。

糖脂稳态失衡是导致肥胖和2型糖尿病等诸多代谢综合征。本课题组的研究发现，糖脂稳态的调节依赖多器官共同作用，不仅有传统信号分子参与细胞间的信息传递，近年来还发现细胞外RNA作为重要的新一类细胞分泌因子RNAkine也参与到糖脂稳态的器官间/细胞间交流过程中。本课题组致力于探究在肥胖、2型糖尿病和肿瘤等病理过程中，细胞外RNA因子对系统性及区域性糖脂稳态调节和影响，重点进行不同病理条件下功能性RNA因子的发现和功能探究，发现新的介导糖脂稳态的分泌因子，完善糖脂稳态调节机制。

病理状态下RNA因子介导的器官间及区域糖代谢调节

2. 糖脂稳态的代际间传递及机制研究。

流行病学调查显示，代谢表型存在明显的代际遗传效应。表观遗传学是介导代谢表型代际遗传的重要机制。本课题组将进一步探究高龄及现代生活等不良环境因素下，糖脂表型的代际遗传现象，并重点以细胞外RNA因子为切入点探究传递机制。

主要方向二：基于细胞外RNA原理的RNAi疾病治疗策略。

    RNAi基因治疗技术自提出以来广受关注，具有重大的应用前景。本课题组致力于开发siRNA在体内高效、无副作用及具有靶向性的递送方式。为重大疾病（例如胶质母细胞瘤、脑卒中）等重大疾病提供潜在的治疗策略。

1.      Jing Li#,Yujing Zhang#, Yangyang Ye#, Dameng Li#, Yuchen Liu, Eunyoung Lee,

Mingliang Zhang, Xin Dai, Xiang Zhang, ShibeiWang, Junfeng Zhang, Weiping Jia, Ke Zen, Antonio Vidal-Puig*, Xiaohong Jiang*, Chen-Yu Zhang*. Pancreatic β cells control glucose homeostasis via the secretion of exosomal miR-29family. J Extracell Vesicles 2021Jan;10(3):e12055.  doi:10.1002/jev2.12055. Epub 2021 Jan 21. 

2.      Yujie Zhong#, Xiaoyu Ding#, Yuying Bian#, Jing Wang, Wanqing Zhou, Xiangdong Wang,

Pumin Li, Yi Shen, Jun-Jun Wang, Jing Li*, Chunni Zhang* and Cheng Wang*. Discovery and validation of extracellular vesicleassociated miRNAs as noninvasive detection biomarkers for early-stage non-small-cell lung cancer. Mol Oncol 2020 Dec 19.doi: 10.1002/1878-0261.12889. 

3.      Dameng Li#, Huichen Song#, Linghu Shuo, Lei Wang, Ping Xie, Weili Li, Jiachen Liu, Yafei

Tong, Chen-Yu Zhang, Xiaohong Jiang*, Jing Li*, Yujing Zhang*.Gonadal white adipose tissue-derived exosomal MiR-222 promotes obesity-associated insulin resistance. 2020 Nov 10;12(22):22719-22743.doi: 10.18632/aging.103891. Epub 2020 Nov 10. IF = 4.831 （2019），分区：Q2

4.      Dameng Li#, Wen Zhang #, Xiulan Chen# , Hushuo Ling , Ping Xie , Zihao Chen , Amila Adili , Zixuan Chen, Fuquan Yang , Chen-Yu Zhang, Xiaohong Jiang*, Jing Li*, Yujing Zhang*. Proteomic profiling of MIN6 cell-derived exosomes. J Proteomics. 2020 Jul 30;224:103841. doi: 10.1016/j.jprot.2020.103841. Epub 2020 May 24. 

5.      Y. Ye#, X. Zhang#, F. Xie, B. Xu, P. Xie, T. Yang, Q. Shi, C. Zhang, Y. Zhang, J. Chen*, X. Jiang* and J. Li*.An engineered exosome for delivering sgRNA:Cas9 ribonucleoprotein complex and genome editing in recipient cells. Biomater. Sci., 2020, DOI: 10.1039/D0BM00427H. 

6.      Zheng S#, Guo S#, Sun G#, Shi Y, Wei Z, Tang Y, He F, Shi C, Dai P, Chong H, Samuelson I, Zen K, Zhang CY, Zhang Y*, Li J*, Jiang X*. Gain of Metabolic Benefit with Ablation of miR-149-3p from Subcutaneous Adipose Tissue in Diet-Induced Obese Mice. Mol Ther Nucleic Acids. 2019 Dec 6;18:194-203. Epub 2019 Aug 26. doi: 10.1016/j.omtn.2019.07.024. 

7.      Wen Zhang, Pei He, Shibei Wang, Amila Adili, Zixuan Chen, Chen-Yu Zhang, Xiaohong Jiang*, Jing Li*, Yujing Zhang*. Characterization of Protein Profiling and mRNA Expression of LLC Exosomes. Protein J. 2019 Oct;38(5):586-597, doi: 10.1007/s10930-019-09849-0. 

8.      Aisen Zhang, Dameng Li, Yucheng Liu, Jing Li*, Yujing Zhang*, Chen-Yu Zhang. Islet β cell: An endocrine cell secreting miRNAs. Biochem Biophys Res Commun. 2018 Jan 8;495(2):1648-1654.doi: 10.1016/j.bbrc.2017.12.028. 

9.     Rui Zhang#, Rui Liu#, Chang Liu#, Yahan Niu, Jianguo Zhang, Baoliang Guo, Chen-Yu Zhang Jing Li*, Jie Yang*, Xi Chen*. A novel role for miR-520a-3p in regulating EGFR expression in colorectal cancer. Cellular Physiology and Biochemistry. 2017 July; 42:1559-1547. DOI:10.1159/000479397. 

10.  Liu Y#, Liu R#, Yang F#, Cheng R, Chen X, Cui S, Gu Y, Sun W, You C, Liu Z, Sun F, Wang Y, Fu Z, Ye C, Zhang C*, Li J*, Chen X*.miR-19a promotes colorectal cancer proliferation and migration by targeting TIA1.Mol Cancer. 2017 Mar 4;16(1):53. doi: 10.1186/s12943-017-0625-8. 

11.  You C#, Liang H#, Sun W#, Li J#, Liu Y, Fan Q, Zhang H, Yue X, Li J*, Chen X*, Ba Y*. Deregulation of the miR-16-KRAS axis promotes colorectal cancer.Sci Rep. 2016 Nov 18;6:37459. doi: 10.1038/srep37459. 

12.  Li J#, Chen X#, Yi J#, Liu Y, Li D, Wang J, Hou D, Jiang X, Zhang J, Wang J, Zen K, Yang F, Zhang CY, Zhang Y. Identification and Characterization of 293T Cell-Derived Exosomes by Profiling the Protein, mRNA and MicroRNA Components. pLoS one. 2016 sep 20:11(9):30163043.doi:10.1371/journal.pone.0163043.eCollection 2016. 

13.  Pan Y#, Li J#, Zhang Y, Wang N, Liang H, Liu Y*, Zhang CY*, Zen K*, Gu H*.Slug-upregulated miR-221 promotes breast cancer progression through suppressing E-cadherin expression.Sci Rep. 2016 May 13;6:25798. doi: 10.1038/srep25798. 

14.  Ding H#, Zheng S#, Garcia-Ruiz D, Hou D, Wei Z, Liao Z, Li L, Zhang Y, Han X, Zen K, Zhang CY*, Li J*, Jiang X*. Fasting induces a subcutaneous-to-visceral fat switch mediated by microRNA-149-3p and suppression of PRDM16.Nat Commun. 2016 May 31;7:11533. doi: 10.1038/ncomms11533.

15.  Liu Y#, Li D#, Liu Z#, Zhou Y, Chu D, Li X, Jiang X, Hou D, Chen X, Chen Y, Yang Z, Jin L, Jiang W, Tian C, Zhou G, Zen K, Zhang J, Zhang Y*, Li J*, Zhang CY*.Targeted exosome-mediated delivery of opioid receptor Mu siRNA for the treatment of morphine relapse.Sci Rep. 2015 Dec 3;5:17543. doi: 10.1038/srep17543.

16.  Xue Y#, Wei Z#, Ding H#, Wang Q, Zhou Z, Zheng S, Zhang Y, Hou D, Liu Y, Zen K, Zhang CY*, Li J*, Wang D*, Jiang X*.MicroRNA-19b/221/222 induces endothelial cell dysfunction via suppression of PGC-1α in the progression of atherosclerosis.Atherosclerosis. 2015 Aug;241(2):671-81. doi: 10.1016/j.atherosclerosis.2015.06.031.

17.  Li J#, Zhang Y#, Li D, Liu Y, Chu D, Jiang X, Hou D, Zen K*, Zhang CY*.Small non-coding RNAs transfer through mammalian placenta and directly regulate fetal gene expression.Protein Cell. 2015 Jun;6(6):391-6. doi: 10.1007/s13238-015-0156-2. No abstract available.

18.  Zhang J#, Li L#, Li J#, Liu Y, Zhang CY#, Zhang Y#, Zen K#.Protein tyrosine phosphatase 1B impairs diabetic wound healing through vascular endothelial growth factor receptor 2 dephosphorylation.Arterioscler Thromb Vasc Biol. 2015 Jan;35(1):163-74. doi: 10.1161/ATVBAHA.114.304705.

19.  Liang H#, Liu M#, Yan X#, Zhou Y#, Wang W, Wang X, Fu Z, Wang N, Zhang S, Wang Y, Zen K, Zhang CY, Hou D*, Li J*, Chen X*.miR-193a-3p functions as a tumor suppressor in lung cancer by down-regulating ERBB4.J Biol Chem. 2015 Jan 9;290(2):926-40. doi: 10.1074/jbc.M114.621409.

20.  Liu Y, Zhao L, Li D, Yin Y, Zhang CY*, Li J*, Zhang Y*.Microvesicle-delivery miR-150 promotes tumorigenesis by up-regulating VEGF, and the neutralization of miR-150 attenuate tumor development.Protein Cell. 2013 Dec;4(12):932-41. doi: 10.1007/s13238-013-3092-z.

21.  Li J#, Zhang Y#, Liu Y, Dai X, Li W, Cai X, Yin Y, Wang Q, Xue Y, Wang C, Li D, Hou D, Jiang X, Zhang J, Zen K*, Chen X*, Zhang CY*.Microvesicle-mediated transfer of microRNA-150 from monocytes to endothelial cells promotes angiogenesis.J Biol Chem. 2013 Aug 9;288(32):23586-96. doi: 10.1074/jbc.M113.489302.