王斌 教授

2009年获美国密歇根大学，生态与进化生物学系博士学位；

2011年-2016年，南京大学生命科学学院 副教授

2017年至今，南京大学生命科学学院 教授

为了适应营养贫乏的陆生环境，植物进化出了与环境中特定微生物形成互惠共生体，从而提高其养分获取的能力。其中，丛枝菌根（Arbuscular Mycorrhiza, AM）是一类起源古老，演化至今仍在近80%陆地植物中普遍发生的共生方式。它不仅能极大地促进植物对磷、氮等矿质营养的吸收，还能有效提高植物对多种生物或非生物胁迫的抗性。研究人员将AM共生的建立过程分为五个阶段：1）在接触之前，植物根系就会分泌独脚金内酯（Strigolactones, SLs）等物质诱导周围真菌孢子萌发和菌丝延伸，真菌也释放菌根因子激活植物根细胞中的共生信号通路；2）真菌接触根表皮后，菌丝前端形成附着结构菌足；3）菌足下方的细胞核发生移动，通过细胞骨架重排形成预穿透通道（Prepenetration apparatus, PPA），菌丝随即沿着PPA穿过表皮和外皮层；4）菌丝到达内部皮层细胞后，逐步经历主干、初级分支、次级分支等发育阶段，最终形成树丛状的共生核心结构—丛枝（Arbuscules），丛枝被植物细胞质膜凹陷所衍生的围丛枝膜（Peri-arbuscular membrane, PAM）紧密包围；植物与真菌在围丛枝膜与丛枝之间的广泛共生界面上，高效进行营养交换：真菌将自身从土壤中吸收的磷、氮等矿质营养输送给植物，植物则将有机碳主要以脂质的形式输送给真菌；5）最终，丛枝逐渐退化，真菌也产生储存脂质的囊泡（Vesicles）和子代孢子，完成其生活史。

我们的研究目标是揭示植物在不同阶段调控丛枝菌根共生的具体分子机制，主要研究对象为单子叶植物水稻和豆科植物苜蓿。具体工作包括针对不同的植物共生响应基因，构建其功能缺失、过表达突变体观察表型变化，同时结合基因的组织表达位置和蛋白亚细胞表达位置，提出相应的分子调控模型；再通过蛋白互作、生化分析等手段加以验证。

告知：我们明年（2026.9入学）已无博士名额，请同学们不用再联系（2025.9）。

Tian L#, Hao YM#, Guo R#, Guo HR#, Cheng JF, Liu TR, Liu H, Lu GH*, Wang B* (2024) Two lysin motif extracellular  (LysMe) proteins are deployed in rice to facilitate arbuscular mycorrhizal symbiosis. New Phytologist doi: 10.1111/nph.19873.

Shan Z, Chu YL, Sun GF,Chen R, Yan J, He QW, Liu YN, Wang B, Luan MD, Lan WZ (2024) Mechanisms of vacuolar phosphate efflux supporting soybean root hair growth in response to phosphate deficiency. J Integr Plant Biol doi: 10.1111/jipb.13735.

Guan LR, Yin L, Liu YN, Yan J, Wang B, Luan MD, Lan WZ (2024) A plasma membrane localized transporter remobilizes aleurone layer magnesium for seed germination in rice. Plant Journal doi: 10.1111/tpj.16867.

Liu YN#, Liu CC#, Guo R, Tian L, Cheng JF, Wu YN, Wang D, Wang B*(2022) The rice Qa-SNAREs in SYP13 subfamily are involved in regulating arbuscular mycorrhizal symbiosis and seed fertility. Frontiers in Plant Science 13: 898286.

Liu CC#, Liu YN#, Cheng JF, Guo R, Tian L, Wang B*(2022) Dual roles of OsGH3.2 in modulating rice root morphology and affecting arbuscular mycorrhizal symbiosis. Frontiers in Plant Science 13: 853435.

Guo R, Wu YN, Liu CC, Liu YN, Tian L, Cheng JF, Pan ZY, Wang D, Wang B* (2022) OsADK1, a noval kinase regulating arbuscular mycorrhizal symbiosis in rice. New Phytologist 234: 256-268.

Liu YN#, Liu CC#, Zhu AQ, Niu KX, Guo R, Tian L, Wu YN, Sun B, Wang B* (2022) OsRAM2 function in lipid biosynthesis is required for arbuscular mycorrhizal symbiosis in rice. Molecular Plant-Microbe Interactions 35: 187-199.

Liu XT#, Wu XY#, Wu WP, Wu M*, Chen JQ*, Wang B* (2021) A bean common mosaic virus-resistance gene in the soybean variant V94-5152 was mapped to the Rsv4 locus conferring resistance to soybean mosaic virus. Theoretical and Applied Genetics 134: 2367-2377.

Yang Z#, Chi XY#, Guo FF, Jin XY, Luo HL, Hawar A, Chen YX, Feng KK, Wang B, Qi JL, Yang YH*, Sun B*(2020) SbWRKY30 enhances the drought tolerance of plants and regulates a drought stress-responsive gene,SbRD19, in Sorghum. Journal of Plant Physiology 246-247: 153142.

Chen TS, Wang B, Wang FF, Niu GT, Zhang S, Li JM, Hong Z* (2020) The evolutionarily conserved serine residues in BRI1 LRR motifs are critical for protein secretion. Frontiers in Plant Science 11: 32.

Wu M#, Liu YN#, Zhang C, Liu XT, Liu CC, Guo R, Niu KX, Zhu AQ, Yang JY, Chen JQ*, Wang B* (2019) Molecular mapping of the genes conferring resistance to Soybean mosaic virus and Bean common mosaic virus in the soybean cultivar Raiden. Theoretical and Applied Genetics 132(10): 3101-3114.

Shao ZQ#, Xue JY#, Wang Q, Wang B, Chen JQ* (2019) Revisiting the origin of plant NBS-LRR genes. Trends in Plant Science 24(1): 9-12.

Xu RR, Yang WD, Niu KX, Wang B*, Wang WM* (2018) An update on the evolution of Glucosyltransferase (Gtf) genes in Streptococcus. Frontiers in Microbiology 9: 2979.

Wu Y, Wu P, Wang B, Shao ZQ* (2018) Genome-wide analysis reveals ancestral lack of seventeen different tRNAs and clade-specific loss of tRNA-CNNs in Archaea. Frontiers in Microbiology 9: 1245.

Wu M, Wu WP, Liu CC , Liu YN, Wu XY, Ma FF, Zhu AQ, Yang JY*, Wang B*, Chen JQ* (2018) A bean common mosaic virus (BCMV)-resistance gene is fine-mapped to the same region as Rsv1-h in the soybean cultivar Suweon 97. Theoretical and Applied Genetics 131(9): 1851-1860.

Ma FF#, Wu M#, Liu YN, Feng XY, Wu XZ, Chen JQ*, Wang B* (2018) Molecular characterization of NBS-LRR genes in the soybean Rsv3 locus reveals several divergent alleles that likely confer resistance to the soybean mosaic virus.Theoretical and Applied Genetics 131(2): 253-265.

Chen YX#, Wu M#, Ma FF, Chen JQ*, Wang B* (2017) Complete nucleotide sequences of seven soybean mosaic viruses (SMV), isolated from wild soybeans (Glycine soja) in China. Archives of Virology 162(3): 901-904.

Ma FF#, Wu XY#, Chen YX, Liu YN, Shao ZQ, Wu P, Wu M, Liu CC, Wu WP, Yang JY, Li DX, Chen JQ*, Wang B* (2016) Fine mapping of the Rsv1-h gene in the soybean cultivar Suweon97 that confers resistance to two Chinese strains of the soybean mosaic virus.Theoretical and Applied Genetics 129: 2227-2236.

Shao ZQ, Wang B*, Chen JQ* (2016) Tracking ancestral lineages and recent expansions of NBS-LRR genes in angiosperms. Plant Signaling & Behavior11(7): e1197470.

Wu XY#, Zhou GC#, Chen YX, Wu P, Liu LW, Ma FF, Wu M, Liu CC, Zeng YJ, Chu AE, Hang YY, Chen JQ*, Wang B*(2016) Soybean cyst nematode resistance emerged via artificial selection of duplicated serine hydroxylmethyltransferase genes. Frontiers in Plant Science 7:998.

Wu P, Wu Y, Liu CC, Liu LW, Ma FF, Wu XY, Wu M, Hang YY, Chen JQ, Shao ZQ*, Wang B* (2016) Identification of arbuscular mycorrhiza (AM)-responsive microRNAs in tomato. Frontiers in Plant Science 7: 429.

Shao ZQ#, Xue JY#, Wu P, Zhang YM, Wu Y, Hang YY, Wang B*, Chen JQ* (2016) Large-scale analyses of angiosperm Nucleotide-Binding Site-Leucine-Rich Repeat (NBS-LRR) Genes reveal three anciently diverged classes with distinct evolutionary patterns. Plant Physiology, 170 (4): 2095-2109.

Pan HR, Oztas O, Zhang XW, Wu XY, Stonoha C, Wang ET, Wang B, Wang D* (2016) A symbiotic SNARE protein generated by alternative termination of transcription. Nature Plants 2：15197.

Zhang YM, Shao ZQ, Wang Q, Hang YY, Xue JY, Wang B*, Chen JQ* (2016) Uncovering the dynamic evolution of nucleotide-binding site-leucine-rich repeat (NBS-LRR) genes in Brassicaceae. J Integra Plant Biol 58(2): 165-177.

Zhou GC, Shao ZQ, Ma FF, Wu P, Wu XY, Xie ZY, Yu DY, Cheng H, Liu ZH, Jiang ZF, Chen QS, Wang B*, Chen JQ* (2015) The evolution of soybean mosaic virus: An updated analysis by obtaining 18 new genomic sequences of Chinese strains/isolates. Virus Research 208: 189-198.

Shao ZQ, Zhang YM, Hang YY, Xue JY, Zhou GC, Wu P, Wu XY, Wu XZ, Wang Q, Wang B*, Chen JQ* (2014) Long-term evolution of nucleotide-binding site-leucine-rich repeat genes: understanding gained from and beyond the legume family. Plant Physiology, 166(1): 217-234.

Zhou GC, Wu XY, Zhang YM, Wu P, Wu XZ, Liu LW, Wang Q, Hang YY, Yang JY, Shao ZQ*, Wang B*, Chen JQ* (2014) A genomic survey of thirty soybean-infecting bean common mosaic virus (BCMV) isolates from China pointed BCMV as a potential threat to soybean production. Virus Research 191: 125-133.

Wu P, Shao ZQ, Wu XZ, Wang Q, Wang B, Chen JQ, Hang YY, Xue JY (2014) Loss/retention and evolution of NBS-encoding genes upon whole genome triplication of Brassica rapa. Gene, 540(1): 54-61.

Zhang YM, Shao ZQ, Yang LT, Sun XQ, Mao YF, Chen JQ*, Wang B* (2013) Non-random arrangement of synonymous codons in archaea coding sequences. Genomics 101 (6) 362-367.

Shao ZQ, Zhang YM, Pan XZ, Wang B*, Chen JQ* (2013) Insight into the evolution of the Histidine Triad Protein (HTP) family in Streptococcus. PLoS ONE 8 (3) e60116.

Xue JY, Wang Y, Wu P, Wang Q, Yang LT, Pan XH, Wang B*, Chen JQ*（2012）A primary survey on bryophyte species revealed two novel classes of nucleotide-binding site (NBS) genes. PLoS ONE 7 (5) e36700.

Shao ZQ, Zhang YM, Feng XY, Wang B*, Chen JQ* (2012) Synonymous Codon Ordering: A Subtle but Prevalent Strategy of Bacteria to Improve Translational Efficiency. PLoS ONE, 7(3): e33547.

Wang B, Shao ZQ, Xu Y, Liu J, Liu Y, Hang YY, Chen JQ (2011) Optimal codon identities in bacteria: Implications from the conflicting results of two different methods. PloS ONE 6 (7) e22714.

Wang B, Yuan J, Liu J, Jin L, Chen JQ (2011) Codon usage bias and determining forces in green plant mitochondrial genomes. J Integra Plant Biol 53: 324-334.

Wang B, Liu J, Jin L, Feng XY, Chen JQ (2010) Complex mutation and weak selection together determined the codon usage bias in bryophyte mitochondrial genomes. J Integra Plant Biol 52: 1100-1108.

Liu Y#, Wang B#, Cui P#, Li LB, Xue JY, Yu J, Qiu YL (2012) The mitochondrial genome of the lycophyte Huperzia squarrosa: the most archaic form in vascular plants. PLoS ONE 7 (4) e35168.

Liu Y, Xue JY, Wang B, Li LB, Qiu YL. (2011) The Mitochondrial Genomes of the Early Land Plants Treubia lacunosa and Anomodon rugelii: Dynamic and Conservative Evolution. PLoS ONE 6 (10) e25836.

Qiu YL, Li L, Wang B, Xue JY, Hendry TA, Li RQ, Brown JW, Liu Y, Hudson GT, Chen ZD (2010) Angiosperm phylogeny inferred from sequences of four mitochondrial genes. J Syst Evol 48: 391-425.

Xue JY, Liu Y, Li L, Wang B, Qiu YL (2010) The complete mitochondrial genome sequence of the hornwort Phaeoceros laevis: retention of many ancient pseudogenes and conservative evolution of mitochondrial genomes in hornworts. Curr Genetics 56: 53-61.

Wang B, Yeun LH, Xue JY, Liu Y, Ane JM, Qiu YL (2010) Presence of three mycorrhizal genes in the common ancestor of land plants suggests a key role of mycorrhizas in the colonization of land by plants. New Phytologist 186: 514-525.

Wang B#, Xue JY#, Li L, Liu Y, Qiu YL (2009) The complete mitochondrial genome sequence of liverwort Pleurozia purpurea reveals extremely conservative mitochondrial genome evolution in liverworts. Curr Genetics 55: 601-609.

Li L#, Wang B#, Liu Y, Qiu YL (2009) The complete mitochondrial genome sequence of hornwort Megaceros aenigmaticus reveals a mixed mode of conservative yet dynamic evolution in early land plant mitochondrial genomes. J Mol Evol. 68: 665-678.

Hendry TA, Wang B, Yang Y, Davis EC, Braggins JE, Schuster RM, Qiu YL (2007) Evaluating phylogenetic positions of four liverworts from New Zealand, Neogrollea notabilis, Jackiella curvata, Goebelobryum unguiculatum and Herzogianthus vaginatus, using three chloroplast genes. Bryologist 110: 738-751.

Qiu YL, Li L, Wang B, Chen ZD, Dombrovska O, Lee J, Kent L, Li R, Jobson RW, Hendry TA, Taylor DW, Testa CM, Ambros M (2007) A Non-flowering land plant phylogeny inferred from nucleotide sequences of seven chloroplast, mitochondrial and nuclear genes. Intl J Plant Sci 168: 691-708.

Qiu YL, Li L, Wang B, Chen ZD, Knoop V, Groth-Malonek M, Dombrovska O, Lee J, Kent L, Rest J, Estabrook GF, Hendry TA, Taylor DW, Testa CM, Ambros M, Crandall-Stotler B, Duff RJ, Stech M, Frey W, Quandt D, Davis CC (2006) The deepest divergences in land plants inferred from phylogenomic evidence. Proc Natl Acad Sci USA103: 15511-15516.

Wang B, Qiu YL (2006) Phylogenetic distribution and evolution of mycorrhizas in land plants. Mycorrhiza 16: 299-363.